Key dates and Program > Workshops (17th-19th Nov 2018)

 Small group for each workshop (max 25 attendants)

Volunteers are very welcome to chair these workshops.

Register by sending an email to marinelipids@sciencesconf.org

If you wish to register to several workshops, please mention an order of preference.

First come first serve

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Saturday 17^th November

Workshop 1 – Sample preservation and storage: associated issues for lipid extraction and analysis

Contacts: Fabrice.Pernet@ifremer.fr & Lydie.couturier@univ-brest.fr

There is a growing interest for determining lipid composition of marine organisms in different fields such as trophic ecology, human nutrition, physiology and biotechnology. Although lipid extraction protocol are relatively standard (Floch 1957, Bligh and Dyer 1959), dedicated studies on the effect of sampling, preservation and storage on lipids are rare. Lipid textbooks usually recommend to store samples at lowest possible temperatures for the shortest time period with antioxidant molecules (BHT) and under nitrogen atmosphere or vacuum. However, logistical limitations of marine sampling (e.g. remote marine station or boat) often prevent reaching these conditions and compromises have to be done. To our knowledge, alternative conditions (and their effect) are rarely published in the method section of scientific articles.

The first objective of this workshop is to present a state of knowledge on the current methods used for preservation and storage during marine sampling and their potential effects on lipid composition. Then, we propose that participants present or discuss their own unpublished experiments dedicated to these issues. Finally, keypoints of the workshop will be compiled and could be used as material for a collaborative and upcoming publication.

Workshop keywords (not exhaustive): Oxidation, degradation, lipid-hydrolysis, freeze-drying, fresh-frozen, lipid extraction solvent, ethanol storage, long-term storage

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Sunday 18^th November

Workshop 2 – Numerical tools for lipid composition data analysis

Contacts: Olivier.Gauthier@univ-brest.fr  &  Lydie.couturier@univ-brest.fr

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Monday 19^th November

        Workshop 3 – Stable isotopes in marine lipid science

Contacts: Gauthier.Schaal@univ-brest.fr & Edouard.kraffe@univ-brest.fr & Loic.Michel@ifremer.fr

Lipids (fatty acids, sterols) are increasingly used in combination with stable isotopes to elucidate biogeochemical, ecological or metabolic fluxes within marine organisms and ecosystems. Beyond comparing lipid composition and bulk stable isotope data, compound-specific isotope analysis (CSIA) (through GC-c-IRMS), sometimes coupled to experimental ¹³C enrichment, allows characterizing the origin of lipid compounds in a diversity of biological matrices. Examples of CSIA uses in marine lipid science range from the elucidation of lipid biosynthesis pathways in marine organisms to the identification of food sources assimilated by marine top-predators, including the characterization of microbial communities.

This workshop will represent a unique opportunity for students and researchers using CSIA and/or experimental ¹³C-enrichment to share their experience regarding sample preparation, analytical methods and data treatment.

 Keywords (non exhaustive): Compound specific isotope analysis, stable isotopes, isotopomics, lipid biosynthesis pathways, trophic ecology

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Monday 19^th November

Workshop 4 – Marine Lipids for medical and biotechnological applications ; targeted vs non targeted approaches 

Contacts: Jose.Zambonino@ifremer.fr & Soudant.philippe@univ-brest.fr

Lipidomic is a newly and rapidly expanding research field greatly facilitated by recent advances in electrospray ionization mass spectrometry (ESI/MS) and tandem mass spectrometry (MS/MS). Lipidomic characterizes and quantifies thousands of distinct constituents of the lipidome without any “a priori” by using multiple techniques, and allows examining changes in the interactions of constituents in the cellular lipidome. However, lot of so called “lipidomic” studies could not be considered as such and should be considered as classical “with a priori” biochemical approaches. Indeed, although using advanced technology (MS techniques) coupled or not with chromatographic methods, they are targeting specific compounds (lipid classes, subclasses and individual molecular species).

 Lipidomics and lipid biochemistry are in fact complementary because the later approach is mostly used in routine for identifying and quantifying compounds revealed by lipidomics.

 These two analytical approaches have been used to :

 -         Understand lipid biosynthesis pathways, physiology and metabolism: by quantifying the precise chemical compounds in cellular lipidome, identifying their subcellular organization and fluxes, unrevealing the biochemical mechanisms through which lipids interact with each other and with cellular proteins…

 -          Detect common fraud by evidencing fingerprints of specific lipid matrixes

 -          Screen matrices and identify potential biologically active metabolites through bio-guided techniques (bioassays).

 These are only a few potential examples of studies to unravel the lipidome in physiologic signaling and medical studies. This workshop aims at sharing examples of implementation of these techniques with a bioprospecting perspective.

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